PHYLOGENETIC GROUPING AND GENETIC PREDICTORS OF BETA-LACTAMASE ACTIVITY OF ESCHERICHIA COLI STRAINS ISOLATED FROM ULCERATIVE COLITIS PATIENTS
https://doi.org/10.17021/2020.15.4.57.66
Abstract
The state of antibiotic resistance at the phenotypic level has been studied, molecular genetic mechanisms leading to resistance to beta-lactam antibiotics in phylogenetic groups of Escherichia coli strains have been considered. The practical part of the study involved the accumulation of a culture museum consisting of 87 strains of Escherichia coli. These strains were isolated from biopsies of the large intestine mucosa of 46 patients with ulcerative colitis who were under treatment or outpatient examination in 2016-2020 at the clinics of Samara State Medical University. According to conventional techniques, the activity of the spectrum of antibacterial drugs presented in the National Clinical Guidelines was evaluated. Using polymerase chain reaction methods, extended-spectrum beta-lactamase genes were indicated, phylogenetic groups were separated, author's software and patent solutions were described.
About the Authors
E. E. Kruglov
Samara State Medical University
Russian Federation
Russian Federation
M. A. Makarova
Saint-Petersburg Pasteur Institute
Russian Federation
Russian Federation
Y. V. Myakisheva
Samara State Medical University
Russian Federation
Russian Federation
A. V. Zhestkov
Samara State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Kruglov E.E., Makarova M.A., Myakisheva Y.V., Zhestkov A.V. PHYLOGENETIC GROUPING AND GENETIC PREDICTORS OF BETA-LACTAMASE ACTIVITY OF ESCHERICHIA COLI STRAINS ISOLATED FROM ULCERATIVE COLITIS PATIENTS. Astrakhan medical journal. 2020;15(4):57-66. (In Russ.) https://doi.org/10.17021/2020.15.4.57.66
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