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NOVEL APPROACH FOR ISOLATION OF LYTIC BACTERIOPHAGES AGAINST STAPHYLOCOCCUS AUREUS
https://doi.org/10.17021/2020.15.4.8.15
Abstract
Strictly lytic or virulent bacteriophages against Staphylococcus aureus belong to order Caudovirales and comprise predominantly two genera: Kayvirus and Rosenblumvirus. Representatives of both taxonomic groups are well known as safe and viable antibacterial agents. Phages of the genus Kayvirus show usually a broad host range activity against clinical isolates of S. aureus. Nevertheless continuous isolation of new phages against these bacteria is required in order to provide sufficient activity for therapy of biofilms or to prevent phage inactivation by neutralizing antibodies. In the present study, we describe an approach for isolation of lytic bacteriophages of the genus Kayvirus by mitomycin C induction of clinical isolates of S. aureus. Our findings suggest the clinical isolates of S. aureus a reach source for lytic bacteriophages. However taking into account the inducibility of the Kayvirus representatives it have to be evaluated a presence of further unknown life cycles for these bacteriophages such as pseudolysogeny.
About the Authors
E. O. Rubalskii
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School; Laboratory of Applied Immunochemistry, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology
Russian Federation
Russian Federation
O. A. Bashkina
Astrakhan State Medical University
Russian Federation
Russian Federation
A. Haverich
Department of Cardiothoracic, Transplantation and Vascular Surgery; Hannover Medical School
Russian Federation
Russian Federation
A. V. Aleshkin
Laboratory of Clinical Microbiology and Biotechnology of Bacteriophages, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology
Russian Federation
Russian Federation
M. A. Samotrueva
Astrakhan State Medical University
Russian Federation
Russian Federation
E. A. Popov
Astrakhan State Medical University
Russian Federation
Russian Federation
C. Kuehn
Cardiothoracic and Transplant Surgeon, Consultant, Leading Surgeon of Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Russian Federation
Russian Federation
R. O. Abdrakhmanova
Astrakhan State Medical University
Russian Federation
Russian Federation
E. G. Dosmukhanova
Astrakhan State Medical University
Russian Federation
Russian Federation
S. Ruemke
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Russian Federation
Russian Federation
C. Salmoukas
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Russian Federation
Russian Federation
M. O. Rubalsky
City Diagnostic Laboratory
Russian Federation
Russian Federation
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Review
For citations:
Rubalskii E.O., Bashkina O.A., Haverich A., Aleshkin A.V., Samotrueva M.A., Popov E.A., Kuehn C., Abdrakhmanova R.O., Dosmukhanova E.G., Ruemke S., Salmoukas C., Rubalsky M.O. NOVEL APPROACH FOR ISOLATION OF LYTIC BACTERIOPHAGES AGAINST STAPHYLOCOCCUS AUREUS. Astrakhan medical journal. 2020;15(4):8-15. (In Russ.) https://doi.org/10.17021/2020.15.4.8.15
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