NOVEL APPROACH FOR ISOLATION OF LYTIC BACTERIOPHAGES AGAINST STAPHYLOCOCCUS AUREUS
https://doi.org/10.17021/2020.15.4.8.15
Аннотация
Strictly lytic or virulent bacteriophages against Staphylococcus aureus belong to order Caudovirales and comprise predominantly two genera: Kayvirus and Rosenblumvirus. Representatives of both taxonomic groups are well known as safe and viable antibacterial agents. Phages of the genus Kayvirus show usually a broad host range activity against clinical isolates of S. aureus. Nevertheless continuous isolation of new phages against these bacteria is required in order to provide sufficient activity for therapy of biofilms or to prevent phage inactivation by neutralizing antibodies. In the present study, we describe an approach for isolation of lytic bacteriophages of the genus Kayvirus by mitomycin C induction of clinical isolates of S. aureus. Our findings suggest the clinical isolates of S. aureus a reach source for lytic bacteriophages. However taking into account the inducibility of the Kayvirus representatives it have to be evaluated a presence of further unknown life cycles for these bacteriophages such as pseudolysogeny.
Об авторах
E. Rubalskii
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School; Laboratory of Applied Immunochemistry, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology
Россия
Россия
O. Bashkina
Astrakhan State Medical University
Россия
Россия
A. Haverich
Department of Cardiothoracic, Transplantation and Vascular Surgery; Hannover Medical School
Россия
Россия
A. Aleshkin
Laboratory of Clinical Microbiology and Biotechnology of Bacteriophages, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology
Россия
Россия
M. Samotrueva
Astrakhan State Medical University
Россия
Россия
E. Popov
Astrakhan State Medical University
Россия
Россия
C. Kuehn
Cardiothoracic and Transplant Surgeon, Consultant, Leading Surgeon of Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Россия
Россия
R. Abdrakhmanova
Astrakhan State Medical University
Россия
Россия
E. Dosmukhanova
Astrakhan State Medical University
Россия
Россия
S. Ruemke
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Россия
Россия
C. Salmoukas
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School
Россия
Россия
M. Rubalsky
City Diagnostic Laboratory
Россия
Россия
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Рецензия
Для цитирования:
., ., ., ., ., ., ., ., ., ., ., . . Астраханский медицинский журнал. 2020;15(4):8-15. https://doi.org/10.17021/2020.15.4.8.15
For citation:
Rubalskii E.O., Bashkina O.A., Haverich A., Aleshkin A.V., Samotrueva M.A., Popov E.A., Kuehn C., Abdrakhmanova R.O., Dosmukhanova E.G., Ruemke S., Salmoukas C., Rubalsky M.O. NOVEL APPROACH FOR ISOLATION OF LYTIC BACTERIOPHAGES AGAINST STAPHYLOCOCCUS AUREUS. Astrakhan medical journal. 2020;15(4):8-15. (In Russ.) https://doi.org/10.17021/2020.15.4.8.15
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