Investigation of antimicrobial activity of probiotic strain Streptococcus salivarius

Antibiotics in the treatment of diseases of Antibiotics in the treatment of oral cavity diseases lead to bacterial multidrug resistance, so it is necessary to search for alternative methods of treatment. A promising direction is the use of probiotics with antimicrobial potential. The aim of the study was to evaluate the antimicrobial activity of probiotic strain Streptococcus salivarius M18 against strains of Escherichia coli, Staphylococcus aureus depending on the cultivation medium. Materials and Methods. Growth parameters of Streptococcus salivarius M18 strain were studied in milk growth medium, streptococcal broth, thioglycol medium (Russia) by means of daily cultivation on a daily basis. medium (Russia) by daily measurement of the optical density of the culture liquids at a wavelength of 600 nm. at a wavelength of 600 nm on a spectrophotometer PowerWave X spectrophotometer (USA) every 24 h. The total observation period was 15 days. Daily antimicrobial activity of the culture fluid was evaluated by disc-diffusion method against Staphylococcus aureus, Escherichia coli strains on Mueller-Hinton agar. The concentration of lactic acid in the culture fluid was assessed using the kit ‘Lactic acid ‒ Olvex’ (Russia). Descriptive statistics, Duncan's criterion and correlation coefficient were used for statistical analysis of the results. correlation. Results. It was established that in the medium of milk growth medium strain Streptococcus salivarius M18 has expressed growth parameters and shows high functional activity. activity. The zone of growth retardation of Staphylococcus aureus when using 3-day culture fluid was 7 ± 1 mm. When the cultivation period was lengthened to 7 days, the growth retardation zone of Staphylococcus aureus was 7 ± 1 mm and that of Escherichia coli was 10±1 mm. After 10 days of incubation in milk growth medium, the culture fluid of Streptococcus salivarius strain inhibited the growth of Staphylococcus aureus (11.7 ± 0.5 mm) and Escherichia coli (13.3 ± 1.1 mm). In thioglycol medium and streptococcal broth, the strain exhibited lower growth properties and did not show antimicrobial activity until 15 days. Conclusion. Antimicrobial activity of Streptococcus salivarius M18 strain depends on the composition of nutrient medium. The most pronounced growth parameters and functional activity were observed in the milk growth medium.

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