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The effect of prebiotic substrates on the growth of Bifidobacterium bifidum in vitro
https://doi.org/10.17021/1992-6499-2025-3-72-77
Abstract
The article is devoted to the influence of substrates with high prebiotic potential on the functional activity of representatives of the normobiota of the large intestine Bifidobacterium bifidum.
Purpose of the study. Comparative assessment of the prebiotic potential of substrates in relation to Bifidobacterium bifidum under in vitro conditions.
Materials and methods. For the study, we took prebiotic objects that have bifidogenic activity and are available for use among the population for preventive and therapeutic purposes. The lyophilizate of the Bifidobacterium bifidum 791 strain reduced on Kitta-Tarozzi medium was used as a test culture; lactulose, pumpkin fiber, psyllium, apple pectin and inulin were used as prebiotic objects. Sowing of a daily culture of test strains of Bifidobacterium bifidum (n = 9) in 1 ml from pre-prepared dilutions 10–7, 10–8, 10–9, 10–10 was carried out in a regenerated Blaurocca nutrient medium. The results were taken into account visually by the presence of typical colonies in the form of strands characteristic of bifidobacteria (CFU/ml).
Results. Taking into account the prebiotic potential of lactulose revealed stimulation of the growth of bifidobacteria in dilutions 10-7 by 5.7 (p=0.009), in dilutions 10-8 and 10-9 – by 6.6 (p = 0.022 and 0.041, respectively), in 10–10 – by 8.0 times (p = 0.0039) (comparison with culture control). Colonies of Bifidobacterium bifidum, when growing in test tubes with lactulose, looked like dense thick volumetric strands.
Conclusion. The study showed the predominance of the prebiotic potential of lactulose and pumpkin fiber in relation to Bifidobacterium bifidum in vitro. These substrates can be recommended for use in the prevention and treatment of dysbiotic disorders provoked by risk factors.
About the Authors
E. P. Kotelevets
Ryazan State Medical University named after academician I. P. Pavlov
Россия
Россия
Elena P. Kotelevets, Cand. Sci. (Med.), Associate Professor of the Department
Ryazan
V. D. Alfimova
Ryazan State Medical University named after academician I. P. Pavlov
Россия
Россия
Valeria D. Alfimova, student
Ryazan
V. V. Biryukov
Ryazan State Medical University named after academician I. P. Pavlov
Россия
Россия
Vladimir V. Biryukov, Cand. Sci. (Med.), Associate Professor of the Department
Ryazan
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Review
For citations:
Kotelevets E.P., Alfimova V.D., Biryukov V.V. The effect of prebiotic substrates on the growth of Bifidobacterium bifidum in vitro. Astrakhan medical journal. 2025;20(3):72-77. (In Russ.) https://doi.org/10.17021/1992-6499-2025-3-72-77
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