Comparative assessment of the quality of acute pancreatitis models by changes in the concentration of enzymes and C-reactive protein in the blood of rats

Acute pancreatitis remains an unsolved problem of abdominal urgent surgery. Therefore, there remains a need for a deep study of the etiology and pathogenesis of acute pancreatitis, including on the basis of modeling acute pancreatitis in the experiment.

The purpose of the study: to conduct a comparative assessment of the quality of acute pancreatitis models by changes in the concentration of enzymes and C-reactive protein in the blood of rats.

Materials and methods. Acute pancreatitis was modeled in an experiment on 20 outbred white laboratory male rats by subcapsular administration of the nonionic detergent Triton X-100, the anionic detergent sodium dodecyl sulfate, or the cationic detergent cetylpyridinium chloride. A similar operation was performed in the control group, but instead of the detergent solution, the animals were administered a physiological solution. On days 1, 5, 9, and 14 from the moment of modeling acute pancreatitis, the activity of alanine aminotransferase, aspartate aminotransferase, alpha-amylase, and Creactive protein was determined in the blood serum of the animals.

Results. It was found that 5 % concentrations of Triton, sodium dodecyl sulfate and cetylpyridinium chloride used to model pancreatitis caused the development of acute aseptic pancreatitis in rats, which was absent in the control group of rats, which was confirmed by the results of studying the dynamics of alanine aminotransferase, aspartate aminotransferase, alpha-amylase and C-reactive protein levels in rat blood serum in all three models. When pairwise comparing groups of rats with different models of acute aseptic pancreatitis, statistically significant differences between the models were not found. However, according to our observations, the model of pancreatitis induced by sodium dodecyl sulfate should be given priority, as it is forty times less toxic than cetylpyridinium chloride.

Conclusion. The study confirmed the importance of developing new models for simulating acute pancreatitis. The models used in the study ensured 100 % survival of the experimental animals, and the selected biochemical parameters confirmed the development of acute aseptic pancreatites in all experimental groups. The results of the study may be useful in studying new drugs for the treatment of pancreatitis.

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