The influence of professional oral hygiene on the indicators of local immunity

Professional oral hygiene is an important part of measures aimed at preventing and maintaining the patient’s health. Local immune response is the first barrier of immune protection capable of preventing infection diseases. The aim of the study - the influence of professional hygiene on the indicators of local immunity has been studied. The study involved two groups of patients: the first group included 19 individuals with chronic caries of varying depths (class I), and the second group comprised 15 individuals with moderate periodontal disease. To evaluate nonspecific oral protection, the index of natural colonization of the buccal epithelium and the activity of microbial adsorption by oral mucosal cells were assessed. Specific immunity was evaluated by measuring the concentration of immunoglobulins in unstimulated saliva. All indicators were recorded both before and after professional oral hygiene procedures. When studying the index of natural colonization of the buccal epithelium, it was found that professional cleaning in both groups led to normalization of the spectrum of bacteria adhered to the epithelium: there was a decrease in the amount of gram-negative microflora, atypical for this biotope, while maintaining a high adhesion index of gram-positive cocci. In persons with caries, before sanation, “unsatisfactory” resistance of oral mucosa was revealed in a third of the examined, after sanation, the number of such patients decreased by 3 times, with “satisfactory” resistance increased by 2 times. In the group with periodontal disease, all patients exhibited “unsatisfactory” mucosal resistance before the hygiene procedures. After sanitation, 20 % “unsatisfactory” resistance persisted. In the majority of patients (80 %), the resistance of the oral mucosa improved to “satisfactory”. The concentration of all classes of immunoglobulins in both groups before sanitation was significantly below the norm, after the measures taken, the indicators increased significantly and reached the level typical for healthy individuals. Carrying out professional cleaning of the teeth leads to the normalization of local immune status even without additional medical immunocorrection.

1. Katola V. M., Komogorceva V. E. The role of the oral microbiome in the development of inflammation and somatic pathology. Byulleten' fiziologii i patologii dykhaniya = Bulletin of physiology and pathology of respiration. 2018; 68: 117-122. doi: 10.12737/article_5b1a069e8a9318.69578013 (In Russ.).

2. Avdeeva M. V., Samoylova I. G., Shcheglov D. S. Pathogenetic aspects of the relationship of infectious diseases of the oral cavity with the development and progression of atherosclerosis and the possibility of their comprehensive prevention. Zhurnal infektologii = Journal of Infectology. 2012; 4 (3): 30-34 (In Russ.).

3. Mamontova T. V., Vesnina L. E., Kaydashev I. P. The microflora of the oral cavity as a factor in the development of diseases of the cardiovascular system. Ukrainskiy meditsinskiy zhurnal = Ukrainian Medical Journal. 2014; 4 (102): 1-8 (In Russ.).

4. Irfan M., Delgado R. Z. R., Frias-Lopez J. The Oral Microbiome and Cancer. Frontiers in Immunology. 2020. 11: 591088. doi: 10.3389/fimmu.2020.591088. PMID: 33193429; PMCID: PMC7645040.

5. Kosyuga S. Yu., Lekomtseva O. V. The role of dental education in the prevention of dental diseases in schoolchildren aged 14. Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy = International Journal of Applied and Fundamental Research. 2018; 5-1: 113-118 (In Russ.).

6. Nikolaev A. I., Tsepov L. M., Makeeva I. M., Vedyaeva A. P. Professional and individual oral hygiene in adults. Moscow: MEDpress-inform; 2018: 192 p. (In Russ.).

7. Lukinykh L. M. Achievements and problems in the prevention and treatment of caries in the white spot stage. Stomatologiya = Dentistry. 2013; 2: 26 (In Russ.).

8. Ulitovskiy S. B. Individual hygienic program for the prevention of dental diseases Moscow: Meditsinskaya kniga; 2003: 292 p. EDN QLFCWN (In Russ.).

9. Paulina Yu. S. Advantages of controlled oral hygiene according to the iTOP system. Byulleten' meditsinskikh internet-konferentsiy = Bulletin of medical Internet conferences. 2020; 10 (6): 187 (In Russ.).

10. Kondratov A. I. Ways to increase the effectiveness of health education in the comprehensive prevention of dental diseases. Thesis of Candidate of Medical Sciences. Moscow; 1990: 237 p. (In Russ.).

11. Bulgakova A. I. Changes in the indicators of local immunity of the gums and oral cavity of patients in the treatment of chronic periodontitis. Parodontologiya = Periodontology. 2002; 1-2 (23): 55-59 (In Russ.).

12. Stvolygin A. V., Kazarina L. N., Kuzin V. B., Lovtsova L. V., Tepaev D. V. The effect of therapeutic and prophylactic toothpaste “mexidol Dent active” on the state of local immunity of the oral cavity in persons with chronic catarrhal gingivitis. Allergologiya i immunologiya = Allergology and Immunology. 2007; 8 (1): 366. EDN HZRHTT (In Russ.).

13. Zubairova G. Sh., Bulgakova A. I., Medvedev Yu. A., Efimov G. E., Valeev I. V. Kaydanek T. V. Changes in the activity of a number of humoral immunity factors in the oral cavity of patients with chronic generalized periodontitis during treatment with local use of probiotic and immunomodulatory. Meditsinskiy vestnik Bashkortostana = Medical Bulletin of Bashkortostan. 2009; 4 (4): 39-42 (In Russ.).

14. Rizaev Zh. A., Nazarova N. Sh. The state of local immunity of the oral cavity in chronic generalized periodontitis. Vestnik nauki i obrazovaniya = Bulletin of Science and Education. 2020; 14-4 (92): 35-40. doi: 10.24411/2312-8089-2020-11410 (In Russ.).

15. Putneva A. S., Mishchenko M. N., Karavaeva T. M., Mudrov V. A., Maksimenya M. V., Drozdova A. M., Tsybikov N. N., Pateyuk A. V. The relationship between the level of 25(OH)D3 in the blood, the amount of antimicrobial peptides in the oral fluid and the intensity of caries in young people. Sibirskoe meditsinskoe obozrenie = Siberian Medical Review. 2022; 2: 62-68. doi: 10.20333/25000136-2022-2-62-68 (In Russ.).

16. Lukinykh L. M., Kitaeva E. V. Evaluation of indicators of secretory immunity of the oral cavity in dental caries in children and adolescents of various ages. Dental Forum. 2009; 4: 23 (In Russ.).

17. Akhkamova T. M., Bulgakova A. I., Medvedev Yu. A., Valeev I. V. The state of local immunity of the oral cavity in the conditions of complex therapy of chronic generalized periodontitis. Meditsinskiy vestnik Bashkortostana = Medical Bulletin of Bashkortostan. 2007; 2 (2): 83-86 (In Russ.).

18. Konstanty-Kalandyk J., Kalandyk-Konstanty A., Kapelak B., Zarzecka J., Drwila R., Kieltyka A., Pi^tek J., Bartus K., Sadowski J. Incomplete oral sanation as a risk factor for elevated leucocytosis and postoperative infection. Kardiologia Polska. 2016; 74 (10): 1167-1173. doi: 10.5603/KP.a2016.0064. Epub 2016 May 10. PMID: 27160176.

19. Minzdravsotsrazvitiya Rossii ot 05.05.2012 n 14-3/10/1-2819 “Metodicheskie rekomendatsii “Okazanie meditsinskoy pomoshchi vzroslomu naseleniyu v tsentrakh zdorovya” (vmeste s metodicheskimi rekomendatsiyami “Okazanie meditsinskoy pomoshchi vzroslomu naseleniyu v tsentrakh zdorovya”, utv. Minzdravsotsrazvitiem Rossii 23.04.2012) = Ministry of Health and Social Development of the Russian Federation from 05.05.2012 N 14-3/10/1-2819 "Methodological recommendations “Provision of medical care to the adult population in health centers” (together with the methodological recommendations “Provision of medical care to the adult population in health centers”, approved by the Ministry of Health and Social Development of Russia on 23.04.2012). URL: sudact.ru/law/pismo-minzdravsotsrazvitiia-rossii-ot-05052012-n-14-3101-2819 (In Russ.).

20. Danilevsky N. F., Belenchuk T. A. Determination of non-specific resistance of the organism by the degree of activity of the reaction of adsorption of microorganisms by epithelial cells of the oral mucosa. Morfologiya. Respublikanskiy mezhvedomstvennyy sbornik = Morphology. Republican interdepartmental collection. Kyiv: Zdorov'ya; 1988; 2: 10-13 (In Russ.).

21. Vasilyeva E. S. Osnovnye aspekty diagnostiki i lecheniya porazheniy slyunnykh zhelez i organov polosti rta u bolnykh khronicheskim pankreatitom = Basic aspects of diagnostics and treatment of lesions of the salivary glands and oral cavity organs in patients with chronic pancreatitis. Abstract of dissertation of Candidate of Medical Sciences. Yekaterinburg; 1995: 23 p. (In Russ.).

22. Abadzhidi M. A., Makhrova T. V., Mayanskaya I. V., Zaslavskaya M. I., Strogova Yu. Yu., Mayanskiy A. N. Bukkalnye epiteliotsity kak instrument kliniko-laboratornykh issledovaniy = Buccal epithelial cells as a tool of clinical and laboratory research. URL: https://www.medicum.nnov.ru/nmj/2003/3-4/23.php (In Russ.).

23. Petrushanko T. A., Chereda V. V., Loban’ G. A. Role of oral cavity colonization resistance in dental caries development. Stomatologiya = Stomatology. 2013; 92 (1): 43-45 (In Russ.).

24. Ratsyuk M. M., Lukinykh L. M., Zelenova E. G. Index of natural colonization of buccal epithelium in the clinic of therapeutic dentistry. Nizhegorodskiy meditsinskiy zhurnal = Nizhny Novgorod Medical Journal. 2003; Dentistry: 65 -66 (In Russ.).

25. Mayanskiy A. N., Abadzhidi M. A., Mayanskaya I. V., Zaslavskaya M. I., Makhrova T. V. Reactivity of buccal epithelial cells: indication of local and general homeostasis disorders (literature review). Klinicheskaya laboratornaya diagnostika = Clinical laboratory diagnostics. 2004; 8: 31-34. EDN OIWZTB. (In Russ.).

26. Polyakova V. O., Pal’tseva E. M, Krulevskiy V. A. Buccal epithelium. New approaches to the molecular diagnosis of socially significant pathology. St. Petersburg: N-L; 2015: 128 p. (In Russ.).

27. McClure R., Massari P. TLR-dependent human mucosal epithelial cell responses to microbial pathogens. Frontiers in Immunology. 2014. 5: 386. doi: 10.3389/fimmu.2014.00386.

28. Lukova O. A., Zaslavskaya M. I., Makhrova T. V., Kropotov V. S., Kitaeva E. V. Expression of toll-like and adhesive receptors on epithelial cells of the oral mucosa in periodontitis. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics. 2020; 65 (10): 645-648. doi: http://dx.doi.org/10.18821/0869-2084-2020-65-10-645-648 (in Russ.).

29. Totolyan A. A. Modern approaches to the diagnosis of immunopathological conditions. Meditsinskaya immunologiya = Medical Immunology. 1999; 1 (1-2): 75-108 (In Russ.).

30. Gubaidullin A. G., Tuigunov M. M., Bulgakov A. K., Savchenko T. A. Osobennosti patogeneza zabolevaniy parodonta, vyzvannykh Porphyromonas gingivalis = Features of the pathogenesis of periodontal diseases caused by Porphyromonas gingivalis. URL: https://elibrary.ru/download/elibrary_32628890_13178841.pdf (In Russ.).

31. Tsarev V. N., Nikolaeva E. N., Ippolitov E. V. Parodontopatogennye bakterii - osnovnoy faktor vozniknoveniya i razvitiya parodontita = Periodontophatogenic bacteria of the main factors of emergence and development of periodontitis. URL: https://elibrary.ru/download/elibrary_32628890_13178841.pdf (In Russ.).

32. Marshall A., Celentano A., Cirillo N., McCullough M., Porter S. Oral keratinocytes synthesize CTACK: A new insight into the pathophysiology of the oral mucosa. Experimental Dermatology. 2018; 27 (2): 207-210. doi: 10.1111/exd.13471. Epub 2017 Dec 18. PMID: 29171092.

33. Inaba E., Uematsu H., Nishiyama Y., Watanabe H., Senpuku H. The role of anti-PAc (361-386) peptide SIgA antibody in professional oral hygiene of the elderly. Gerodontology. 2009; 26 (4): 259-267. doi: 10.1111/j.1741-2358.2009.00276.x. Epub 2009 Feb 13. PMID: 19260991.