DEVELOPMENT AND TESTING TEST SYSTEMS FOR THE EARLY DETECTION OF BURKHOLDERIA CEPACIA COMPLEX DNA IN SPUTUM BY POLYMERASE CHAIN REACTION IN CYSTIC FIBROSIS
https://doi.org/10.17021/2019.14.3.71.79
Abstract
Burkholderia cepacia complex bacteria are the most prognostically adverse pathogens in cystic fibrosis. Strains are characterized by pronounced transmissibility and ability to spread among patients. Early detection of these bacteria is a necessary condition for eradication and prevention of spread in a hospital. The study of sputum by polymerase chain reaction is included in the list of examinations of patients with cystic fibrosis in Europe, but is not used in routine practice in Russia yet. Development and approbation of 2 test systems for early detection of Burkholderia cepacia complex DNA in sputum by polymerase chain reaction was carried out. The results allow to speak of implementing these studies in the routine practice of laboratories of cystic fibrosis centers in the Russian Federation.
About the Authors
O. V. Kondratenko
Samara State Medical University
Russian Federation
Russian Federation
D. A. Viktorov
TestGene LLC
Russian Federation
Russian Federation
A. N. Toropovskiy
TestGene LLC
Russian Federation
Russian Federation
Y. V. Nazarova
TestGene LLC
Russian Federation
Russian Federation
A. V. Zhestkov
Samara State Medical University
Russian Federation
Russian Federation
D. F. Sergienko
Astrakhan State Medical University
Russian Federation
Russian Federation
S. A. Krasovskiy
Research Institute of Pulmonology; Medico-Genetic Scientific Center named after academician N. P. Bochkov
Russian Federation
Russian Federation
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Review
For citations:
Kondratenko O.V., Viktorov D.A., Toropovskiy A.N., Nazarova Y.V., Zhestkov A.V., Sergienko D.F., Krasovskiy S.A. DEVELOPMENT AND TESTING TEST SYSTEMS FOR THE EARLY DETECTION OF BURKHOLDERIA CEPACIA COMPLEX DNA IN SPUTUM BY POLYMERASE CHAIN REACTION IN CYSTIC FIBROSIS. Astrakhan medical journal. 2019;14(3):71-79. (In Russ.) https://doi.org/10.17021/2019.14.3.71.79
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