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Microecological state of the gut after correction of stress-induced dysbiosis by peptide Thr-Lys-Pro-Arg-Pro-Gly-Pro

https://doi.org/10.17021/1992-6499-2024-3-30-37

Abstract

Stress can disrupt bidirectional communication within the framework of the microbiota-gut-brain axis, including affecting the microbiological landscape and morphofunctional state of the mucous membrane of the colon. In this work we studied the ability of the peptide Thr-Lys-Pro-Arg-Pro-Gly-Pro (tuftsin-pgp) to correct the state of stress-induced dysbiosis in doses of 80, 250 and 750 pg/kg. The increase in the specific content, frequency of occurrence and relative average value of opportunistic bacteria of control stressed animals noted in the study with a decrease in the number and proportion of obligate bacteria indicated active colonization of the mucous membrane of the colon by them. Administration of tuftsin-pgp dose-dependently changed the qualitative and quantitative composition of the studied biotope microbiota. We found out that the most pronounced positive effect on the signs of stress-induced dysbiosis was the administration of the peptide at a dose of 250 pg/kg.

About the Authors

M. S. Krivoshlykova
Kursk State Medical University
Russian Federation

Marina S. Krivoshlykova - Assistant, Kursk State Medical University.

Kursk



O. A. Medvedeva
Kursk State Medical University
Russian Federation

Ol’ga A. Medvedeva - Dr. Sci. (Biol.), Professor, Head of the Department, Kursk State Medical University.

Kursk



I. I. Bobyntsev
Kursk State Medical University
Russian Federation

Igor’ I. Bobyntsev - Dr. Sci. (Med.), Professor, Head of the Department, Director, Kursk State Medical University.

Kursk



A. Yu. Mukhina
Kursk State Medical University
Russian Federation

Aleksandra Yu. Mukhina - Cand. Sci. (Med.), Associate Professor of the Department, Senior Researcher, Kursk State Medical University.

Kursk



A. O. Vorvul’
Kursk State Medical University
Russian Federation

Anton O. Vorvul’ - Cand. Sci. (Med.), Assistant, Senior Researcher, Kursk State Medical University.

Kursk



A. V. Shevchenko
Kursk State Medical University
Russian Federation

Alina V. Shevchenko - Cand. Sci. (Med.), Associate Professor of the Department, Kursk State Medical University.

Kursk



L. A. Andreeva
Kurchatov Institute
Russian Federation

Lyuidmila A. Andreeva - Cand. Sci. (Chem.), Deputy Head of the Laboratory, Kurchatov Institute.

Moscow



N. F. Myasoedov
Kurchatov Institute
Russian Federation

Nikolay F. Myasoedov - Dr. Sci. (Chem.), Academician of RAS, Head of the Laboratory, Kurchatov Institute.

Moscow



References

1. Foster J. A., McVey Neufeld K. A. Gut-brain axis: how the microbiome influences anxiety and depression. Trends in Neurosciences. 2013; 36: 305-312.

2. Sudo N. Effects of gut microbiota on stress response and behavioral phenotype of the host. Brain Nerve. 2016, 68 (6): 595-605.

3. Kozlovskiy I. I., Andreeva L. A., Kozlovskaya M. M., Nadorova A. V., Kolik L. G. On the role of the opioid system in the further anxiolytic effect of the peptide drug Selank. Eksperimental’naya i klinicheskaya farmakologiya = Experimental and Clinical Pharmacology. 2012, 75 (2): 10-13 (In Russ.).

4. Petrovskiy A. K., Petrovskaya A. Yu., Kosenko M. V., Andreeva L. A., Smirnov N. A., Fedorov V. N. Adaptogenic activity of Semax and Selank: experimental study. Meditsinskiy al’manakh = Medical Al-Manakh. 2017; 1: 114-118 (In Russ.).

5. Khavinson V. Kh. Peptide medicines: past, present, future. Klinicheskaya meditsina = Clinical Medicine (Russian Journal). 2020; 98 (3): 165-177 (In Russ.).

6. Mukhina A. Yu., Medvedeva O. A, Svishcheva M. V., Shevchenko A. V., Efremova N. N., Bobyntsev I. I., Kalutskiy P. V. Assessment of the state of microbiocenosis of the large intestine of experimental animals under conditions of immobilization stress. Astrakhanskiy meditsinskiy zhurnal = Astrakhan Medical Journal. 2019; 1: 54-60 (In Russ.).

7. Mukhina A. Y., Medvedeva O. A., Svishcheva M. V., Shevchenko A. V., Efremova N. N., Bobyntsev I. I., Kalutskii P. V., Andreeva L. A., Myasoedov N. F. State of Colon Microbiota in Rats during Chronic Restraint Stress and Selank Treatment. Bulletin of Experimental Biology and Medicine. 2019; 167 (2): 226-228.

8. Bobyntsev I. I., Krivoshlykova M. S., Medvedeva O. A., Vorvul A. O., Andreeva L. A., Myasoedov N. F. State of nervous system functions of Wistar rats when using peptide taftsin-PGP (selank) in restraint stress. Humans and Their Health. 2023; 26 (3): 58-68.

9. Kim Mun Hee, Yea Hyun Leem. Chronic exercise improves repeated restraint stress-induced anxiety and depression through 5HT1A receptor and cAMP signaling in hippocampus. Journal of Exercise Nutrition & Biochemistry. 2014; 18 (1): 97-104.

10. Efimov B. A., Kafarskaia L. I., Korshunov V. M. Study of the parietal microflora in the rat intestine. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology Epidemiology Immunobiology. 2002; 4: 72 -78 (In Russ.).

11. Magurran E. Ekologicheskoe raznoobrazie i ego izmerenie = Ecological diversity and its measurement. Moscow: Mir; 1992: 184 p. (In Russ.).

12. V ’yunova T. V., Andreeva L. A., Shevchenko K. V., Shevchenko V. P., Myasoedov N. F. Peptide regulation of specific ligand-receptor interaction of GABA with the plasma membranes of nerve cells. Neyrokhimiya = Neurochemistry. 2014; 31 (4): 300-306 (In Russ.).

13. Andreeva L. A., Mezentseva M. V., Narovlianskiy A. N., Nagaev I. J., Shapoval I. M., Scherbenko V. E., Russu L. I., Miasoedov N. F. The perspectives of development of new peptide preparations for clinical use which have anti-infection and immune-modulating activity. Infektsiya i immunitet = Russian Journal of Infection and Immunity. 2011, 1 (2): 171-176 (In Russ.).

14. Kolomin T. A., Agapova T. Yu., Agniullin Ya. V., Shram S. I., Shadrina M. I., Slominskiy P. A., Limborskaia S. A., Myasoedov I. F. Transcriptome alteration in hippocampus under the treatment of tuftsin analog Selank. Zhurnal vysshey nervnoy deyatelnosty imeni I. P. Pavlova = Journal of Higher Nervous Activity named after I. P. Pavlov. 2013; 63 (3): 365-374 (In Russ.).

15. Uchakina O. N., Uchakin P. N., Miasoedov N. F., Andreeva L. A., Scherbenko V. E., Mezentseva M. V., Gabaeva M. V., Sokolov O. Yu., Zozulia A. A., Ershov F. I. Immunomodulatory effects of selank in patients with anxiety-asthenic disorders. Zhurnal nevrologii i psikhiatrii imeni S. S. Korsakova = Neuroscience and Behavioral Physiology. 2008, 108 (5): 71 -75 (In Russ.).

16. Grigor’ev S. E. Korrektsiya sindroma postsplenektomicheskogo giposplenizma taftsinsoderzhashchimi preparatami v rannem po-sleoperatsionnom periode (eksperimentalnoe issledovanie) = Correction of Postsplenectomy Hyposplenism Syndrome with Tuftsin-Containing Drugs in the Early Postoperative Period (Experimental Study). Dissertation of Candidate of Medical Sciences. Tomsk; 2013: 139 p. (In Russ.).

17. Vasil’eva L. S., Molokov V. D., Ivanova N. I., Ivanov K. B. Effects of local application of anxiolytic in the complex treatment of experimental periodontitis. Sibirskiy Meditsinskiy Zhurnal (Irkutsk) = Siberian Medical Journal (Irkutsk). 2014; 126 (3): 55-58 (In Russ.).


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Krivoshlykova M.S., Medvedeva O.A., Bobyntsev I.I., Mukhina A.Yu., Vorvul’ A.O., Shevchenko A.V., Andreeva L.A., Myasoedov N.F. Microecological state of the gut after correction of stress-induced dysbiosis by peptide Thr-Lys-Pro-Arg-Pro-Gly-Pro. Astrakhan medical journal. 2024;19(3):30-37. (In Russ.) https://doi.org/10.17021/1992-6499-2024-3-30-37

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ISSN 1992-6499 (Print)