Microbiota of semen samples with asthenozoospermia: analysis of real-time PCR data
https://doi.org/10.17021/1992-6499-2024-3-22-29
Abstract
This study was conducted to compare the semen microbial composition using real-time PCR in patients with asthenozoospermia and normozoospermia. The semen microbial composition was assessed by real-time PCR (Androflor kit, DNA-Technology, Russia) in 242 patients with asthenozoospermia (AS) and 377 patients with normozoospermia (NS). The microbial DNA (as total bacterial load, TBL) in quantity of at least 103 GE/ml was detected in 268 (71.1 %) of 377 semen samples with NS and 185 (76.4 %) of 242 semen samples with AS. According to cluster analysis, the microbiota of positive samples was represented by one of eight stable microbial clusters with predominance of a certain group of bacteria. In asthenozoospermia a cluster dominated by Lactobacillus spp. was determined significantly more often, and among the identified individual groups of bacteria Bacteroides spp. / Porphyromonas spp. / Prevotella spp., Megasphaera spp. / Veillonella spp. / Dialister spp., and Lactobacillus spp. were detected significantly more often in comparison with normozoospermia.
About the Authors
E. S. Voroshilina
Ural State Medical University; Medical Center “Garmonia”
Russian Federation
Russian Federation
Ekaterina S. Voroshilina - Dr. Sci. (Med.), Associate Professor, Professor of the Department, Ural State Medical University; Clinical Laboratory Diagnostician, Head of the Laboratory, “Garmonia” Medical Center.
Yekaterinburg
D. L. Zornikov
Ural State Medical University
Russian Federation
Russian Federation
Danila L. Zornikov - Cand. Sci. (Med.), Associate Professor of the Department, Ural State Medical University.
Yekaterinburg
A. V. Ivanov
Institute of Mathematics and Mechanics
Russian Federation
Russian Federation
Alexey V. Ivanov - Mathematician, Institute of Mathematics and Mechanics.
Yekaterinburg
E. A. Panacheva
Ural State Medical University; Medical Center “Garmonia”
Russian Federation
Russian Federation
Evgeniya A. Panacheva - graduate student of the department microbiology, virology and immunology, Ural State Medical University; Clinical Laboratory Diagnostician, “Garmonia” Medical Center.
Yekaterinburg
V. M. Petrov
Ural State Medical University
Russian Federation
Russian Federation
Vasiliy M. Petrov - Cand. Sci. (Med.), Associate Professor of the Department, Ural State Medical University.
Yekaterinburg
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For citations:
Voroshilina E.S., Zornikov D.L., Ivanov A.V., Panacheva E.A., Petrov V.M. Microbiota of semen samples with asthenozoospermia: analysis of real-time PCR data. Astrakhan medical journal. 2024;19(3):22-29. (In Russ.) https://doi.org/10.17021/1992-6499-2024-3-22-29
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