STUDY OF THE EFFECT OF ASTRAGALUS VULPINUS HERB EXTRACT ON FREE RADICAL PROCESSES IN THE LIVER AND MYOCARDIUM OF WHITE RATS UNDER INFORMATION STRESS

The study aimed to study the effect of the extract of the herb Astragalus vulpinus on the activity of oxidative modification of lipids and proteins in the liver and myocardium tissues of white rats under information stress.
Materials and methods. The experiments were carried out on nonlinear male rats, which were divided into groups (n = 10): I – control individuals receiving distilled water in equi–volume; II – rats with an information stress model; III – animals receiving Astragalus vulpinus extract intragastrically at a dose of 50 mg/kg/day for two weeks and exposed to information stress. Information stress was modelled by forming food-producing behaviour in a multi-alternative maze. After the animals were removed from the experiment, the liver and heart were prepared for subsequent homogenization, tissue extraction and biochemical analysis. To confirm the development of stress, the mass of the adrenal glands was determined, the number of eosinophils in the peripheral blood was calculated and the gastric mucosa was examined to identify erosive and ulcerative formations. The assessment of lipid peroxidation processes was carried out by spectrophotometric measurement of the initial level of TBA-reactive products in liver and myocardial tissue homogenates, the rates of spontaneous and ascorbate- and iron-ion-induced lipid peroxidation. When determining the degree of protein peroxidation in liver and myocardial tissue homogenates, a method based on the reaction of the interaction of oxidized amino acid residues of proteins with 2,4-dinitrophenylhydrazone was used. The state of antioxidant protection was judged by the activity of the catalase enzyme in the studied organ tissues.
Results. During the study, it was revealed that the information load is accompanied by the activation of free radical oxidation processes, which is manifested by an increase in the concentration of products of this process and the level of catalase activity. Analysis of the effect of fox Astragalus extract at a dose of 50 mg/kg on free radical processes in the liver and myocardial tissue under conditions of informational stress showed the presence of pronounced stress-protective and antioxidant properties of this extract, characterized by a change in the intensity of lipid and protein peroxidation processes, as well as the restoration of the level of catalase activity in the studied tissues.
Conclusion. According to the results of biochemical studies, it was found that the use of Astragalus vulpinus herb extract under conditions of informational stress causes its pronounced stress-protective, antioxidant and antiradical properties for the parameters of lipid peroxidation and oxidative modification of proteins, as well as catalase activity in the liver and myocardium tissue of white rats. 

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