ORIGINAL INVESTIGATIONS Original article MOLECULAR EPIDEMIOLOGY OF BURKHOLDERIA CEPACIA COMPLEX STRAINS ISOLATED FROM PATIENTS WITH CYSTIC FIBROSIS IN THE RUSSIAN FEDERATION
https://doi.org/10.48612/agmu/2022.17.2.29.35
Abstract
Molecular characteristics of Burkholderia cepacia complex isolates isolated from Russian patients with cystic fibrosis are presented, including their clonal affiliation and carrying adaptive resistance genes The most prevalent species of Burkholderia cepacia complex recovered from cystic fibrosis patients was Burkholderia cenocepacia (83 %). Burkholderia strains displayed a high level of clonal diversity - ten sequence types were detected among examined isolates. Three new Burkholderia cenocepacia sequence types were discovered.
About the Authors
Y. A. Bocharova
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
A. V. Zhestkov
Samara State Medical University
Russian Federation
Russian Federation
A. V. Lyamin
Samara State Medical University
Russian Federation
Russian Federation
O. V. Kondratenko
Samara State Medical University
Russian Federation
Russian Federation
T. A. Savinova
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
S. V. Polikarpova
Filatov city medical hospital # 15
Russian Federation
Russian Federation
N. I. Fedorova
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
N. A. Mayansky
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
I. V. Chebotar
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
References
1. Jones A. M., Dodd M. E., Govan J. R., Barcus V., Doherty C. J., Morris J., Webb A. K. Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis // Thorax. 2004. Vol. 59, no. 11. P. 948-951.
2. Nash E. F., Thomas A., Whitmill R., Rashid R., Barker B., Rayner R. J., Whitehouse J. L., Honeybourne D. «Cepacia syndrome» associated with Burkholderia cepacia (genomovar I) infection in an adolescent with cystic fibrosis // Pediatric pulmonology. 2011. Vol. 46, no. 5. P. 512-514.
3. Lynch J. P. Burkholderia cepacia complex: impact on the cystic fibrosis lung lesion // In Seminars in respiratory and critical care medicine. 2009. Vol. 30, no. 5. P. 596-610.
4. Whiteford M. L., Wilkinson J. D., McColl J. H., Conlon F. M., Michie J. R., Evans T. J., Paton J. Y. Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak // Thorax. 1995. Vol. 50, no. 11. P. 1194-1198.
5. Drevinek P., Mahenthiralingam E. Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence // Clinical Microbiology and Infection. 2010. Vol. 16, no. 7. P. 821-830.
6. Данные с сайта Всероссийской ассоциации для больных муковисцидозом. Регистр больных муковисцидозом в РФ. URL:https://mukoviscidoz.org/doc/registr/site_Registre_2019.pdf.
7. Bankevich A., Nurk S., Antipov D., Gurevich A. A., Dvorkin M., Kulikov A. S., Lesin V. M., Nikolenko S. I., Pham S., Prjibelski A. D., Pyshkin A. V., Sirotkin A. V., Vyahhi N., Tesler G., Alekseyev M. A., Pevzner P. A. SPAdes: a new genome assembly algorithm and its applications to singlecell sequencing // Journal of Computational Biology. 2012. Vol. 19, no. 5. P. 455-477
8. Lee I., Chalita M., Ha S. M., Na S. I., Yoon S. H., Chun J. ContEst16S: an algorithm that identifies contaminated prokaryotic genomes using 16S RNA gene sequences // International Journal of Systematic and Evolutionary microbiology. 2017. Vol. 67, no. 6. P. 2053-2057.
9. Parks D. H., Imelfort M., Skennerton C. T., Hugenholtz P., Tyson G. W. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes // Genome Research. 2015. Vol. 25, no. 7. P. 1043-1055.
10. Gurevich A., Saveliev V., Vyahhi N., Tesler G. QUAST: quality assessment tool for genome assemblies // Bioinformatics. 2013. Vol. 29, no. 8. P. 1072-1075.
11. Tabacchioni S., Ferri L., Manno G., Mentasti M., Cocchi P., Campana S., Ravenni N., Taccetti G., Dalmastri C., Chiarini L., Bevivino A., Fani R. Use of the gyrB gene to discriminate among species of the Burkholderia cepacia complex // FEMS Microbiology Letters. 2008. Vol. 281, no. 2. P. 175-182.
12. Cystic Fibrosis Foundation 2019 report. URL: https://www.cff.org/Research/Researcher-Resources/Patient-Registry/2019-Cystic-Fibrosis-Foundation-Patient-Registry-Snapshot
13. European Cystic Fibrosis Society Patient Registry Annual Data report 2018. URL: https://www.ecfs.eu/sites/default/files/general-content-files/working-groups/ecfspatientregistry/ECFSPR_Report_2018_v1.4.pdf.
14. Воронина О. Л., Чернуха М. Ю., Шагинян И. А., Кунда М. С., Аветисян Л. Р., Орлова А. А., Лунин В. Г., Авакян Л. В., Капранов Н. И., Амелина Е. Л., Чучалин А. Г., Гинцбург А. Л. Характеристика генотипов штаммов Burkholderia cepacia complex, выделенных от больных в стационарах Российской Федерации // Молекулярная генетика, микробиология и вирусология. 2013. № 2. С. 22-30.
15. Кондратенко О. В. Гетерогенность популяции штаммов рода Burkholderia, выделенных от пациентов с муковисцидозом в Российской Федерации // Иммунопатология, аллергология, инфектология. 2019. № 3. С. 71-74.
16. Cipolla L., Rocca F., Martinez C., Aguerre L., Barrios R., Prieto M. Prevalence of Burkholderiacepacia complex species in cystic fibrosis patients in Argentina during the period 2011-2015 // Enfermedades infecciosas y microbiologia clinica (English Edition). 2018. Vol. 36, no. 7. P. 431-434.
17. Kenna D. T. D., Lilley D., Coward A., Martin K., Perry C., Pike R., Hill R., Turton J. F. Prevalence of Burkholderia species, including members of Burkholderia cepacia complex, among UK cystic and noncystic fibrosis patients // Journal of Medical Microbiology. 2017. Vol. 66, no. 4. P. 490-501.
18. Silva I. N., Santos P. M., Santos M. R., Zlosnik J. E., Speert D. P., Buskirk S. W., Bruger E. L., Waters C. M., Cooper V. S., Moreira L. M. Long-Term Evolution of Burkholderia multivorans during a Chronic Cystic Fibrosis Infection Reveals Shifting Forces of Selection // mSystems. 2016. Vol. 1, no. 3. e00029-16.
19. Domingues S., da Silva G. J., Nielsen K. M.Integrons: Vehicles and pathways for horizontal dissemination in bacteria // Mobile Genetic Elements. 2012. Vol. 2, no. 5. P. 211-223.
20. Heuer H., Binh C. T., Jechalke S., Kopmann C., Zimmerling U., Krögerrecklenfort E., Ledger T., Gonzalez B., Top E., Smalla K. IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversi- fication Driven by Class 1 Integron Gene Cassettes //Frontiers in Microbiology. 2012. Vol. 3. P. 2.
Review
For citations:
Bocharova Y.A., Zhestkov A.V., Lyamin A.V., Kondratenko O.V., Savinova T.A., Polikarpova S.V., Fedorova N.I., Mayansky N.A., Chebotar I.V. ORIGINAL INVESTIGATIONS Original article MOLECULAR EPIDEMIOLOGY OF BURKHOLDERIA CEPACIA COMPLEX STRAINS ISOLATED FROM PATIENTS WITH CYSTIC FIBROSIS IN THE RUSSIAN FEDERATION. Astrakhan medical journal. 2022;17(2):29-35. (In Russ.) https://doi.org/10.48612/agmu/2022.17.2.29.35
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