ORIGINAL INVESTIGATIONS Original article STUDY OF THE EFFECT OF GEL CONTAINING FLAVONOID COMPLEX ON CHANGES IN VASCULAR PERMEABILITY IN RATS WITH EXPERIMENTAL CHRONIC VENOUS INSUFFICIENCY

Abstract

The aim of the study was to evaluate the degree of influence of the gel containing the flavonoid complex on the change in vascular permeability in rats with experimental chronic venous insufficiency. Materials and methods. The study was performed on 66 Wistar female rats weighing 200-220 grams. The animals were obtained from the nursery of laboratory animals “Rappolovo” (Leningrad region). Determining the magnitude of vascular permeability was evaluated in the Miles test. The content of tight junction proteins in the vascular wall was also evaluated. Research results. In rats treated with the analyzed dosage form, vascular permeability was lower than that in the negative control group of rats by 33,4 % (p < 0,05) and by 20,8 % (p < 0,05) compared with animals treated with comparator drug. It was found that the analyzed dosage form, to a comparable extent with the reference drug, eliminates congestion in the venous system, as evidenced by the restoration of blood flow in rats. In addition, the use of the studied gel contributed to a decrease in vascular permeability and an increase in the content of tight junction proteins, occludin and claudin, in the vascular wall, statistically significantly surpassing the reference preparations in efficiency.