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CHANGES IN PHASE COMPOSITION OF PELVIC BIOMINERAL IN JUVENILE RATS WITH STREPTOZOTOCIN-INDUCED DIABETES AFTER PERFORATION OF TIBIA
https://doi.org/10.17021/2021.16.4.24.29
Abstract
The study aims to investigate changes in phase contents of bone mineral of the hipbone in juvenile streptozotocin-induced diabetic rats after surgical perforation of the tibia. Material and methods. Diabetes (35 animals with a weight of 135-150 g at the age of 3 months) was induced by a single intraperitoneal injection of streptozotocin in a dosage of 55 mg per kg of body weight. Surgical perforation of the tibia was modelled as 2 mm through the opening in the proximal metadiaphysis (35 animals). Another group comprised 35 animals with both perforation and diabetes. 35 intact animals comprised the control group. Using the method of internal control, the content in bone mineral of the hipbone whitlockite, calcite and hydroxylapatite was determined. Results. Surgical perforation of the tibia results in instability of phase contents of bone mineral of the hipbone; manifestations peak here was registered on the 30th day of observation. In diabetic animals destabilization of the phase composition of bone, minerals progressed during the experiment. By the 90th day shares of whitlockite and calcite exceeded those of the controls by 13,33 % and 6,77 % while the share of hydroxylapatite decreased by 4,75 %. Surgical perforation of the tibia in diabetic animals resulted in the more marked increase of bone mineral of the hipbone amorphousness from the 60th day of observation; by the 90th-day shares of whitlockite this in rats with perforation by 6,03 % while hydroxylapatite share decreased by 2,18 %. Conclusion. Surgical perforation of the tibia in diabetic juvenile rats increases the amorphousness of bone mineral of the hipbone, which grows beginning from the 60th day after the operation.
About the Author
A. V. Torba
Luhansk State Medical University named after St. Luke
Russian Federation
Russian Federation
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For citations:
Torba A.V. CHANGES IN PHASE COMPOSITION OF PELVIC BIOMINERAL IN JUVENILE RATS WITH STREPTOZOTOCIN-INDUCED DIABETES AFTER PERFORATION OF TIBIA. Astrakhan medical journal. 2021;16(4):24-29. (In Russ.) https://doi.org/10.17021/2021.16.4.24.29
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