THE EFFECT OF GLYPROLINES ON SERUM LEVEL OF BRAIN NEUROTROPHIC FACTOR (BDNF) UNDER CONDITIONS OF “SOCIAL” STRESS

Abstract

Objective: To study the effect of glyprolinesThr-Lys-Pro-Arg-Pro-Gly-Pro (Selank), Pro-Gly-Pro and Pro-Gly-Pro-Leu on the level of Brain-Derived Neurotrophic Factor (BDNF) in serum blood of white rats under conditions of “social” stress. Materials and methods. The study was carried out on 90 male white rats of 6 months of age. Sensory contact with the subsequent formation of aggressive and submissive types of behaviour was used as an experimental model of “social” stress. Laboratory animals, taking into account the gradation by types of behaviour, were divided into groups (n = 10): a group of control / intact animals; a group of rats exposed to “social” stress for 20 days (stress); groups of individuals who received the compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro (Selank), Pro-Gly-Pro, Pro-Gly-Pro-Leu at a dose of 100 μg / kg/day intraperitoneally from the 1st day of stress exposure to a course of 20 days. The BDNF level in the blood serum of white rats was assessed by the enzyme-linked immunosorbent assay using ELISA Kit for Brain-Derived Neurotrophic Factor (USA). Results and conclusion. It was found that under the conditions of “social” stress, a decrease in the level of BDNF was observed. The introduction of the studied glyprolines (Thr-Lys-Pro-Arg-Pro-Gly-Pro (Selank), Pro-Gly-Pro, Pro-Gly-Pro-Leu) under conditions of induced “social” stress increases the concentration of BDNF in the blood serum of experimental animals which indicates the effect of neuropeptides on the level of neurotrophins of the BDNF class and suggests a possible mechanism of the antistress effect.