Resistance of staphylococcal biota of medical staff of a multidisciplinary hospital to bacteriophages

The medical staff of multidisciplinary medical and preventive organizations represents a special category of the population that is involved in the epidemic process of circulation of hospital strains. Their microbiota acquires special biological properties due to constant contact with disinfectants and antibacterial agents, therefore it is relevant to assess the lytic activity of commercial bacteriophage preparations in relation to the microbiota of medical workers. The aim of the study determination of the frequency and spectrum of resistance to commercial bacteriophages of representatives of the genus Staphylococcus isolated from the oropharynx and intestines of medical staff of a multidisciplinary hospital. Materials and methods. The assessment of the lytic activity of commercial bacteriophages “Staphylococcal bacteriophage”, “Pyobacteriophage polyvalent purified”, “Sextaphage pyobacteriophage polyvalent”, “Intesti-bacteriophage” to 130 cultures of staphylococcus isolated from the intestines and throat of the medical staff of a multidisciplinary hospital: S. epidermidis (n = 54), S. haemolyticus (n = 44), S. aureus (n = 32). The lytic activity of bacteriophages was studied using the Appelman method, and the sensitivity of cultures to bacteriophages was studied using the drop method on the Mueller-Hinton medium. Results. Among Staphylococcus epidermidis coproisolytes, strains resistant to 4 bacteriophages were 2 times more common (p = 0.01). Coproisolates showed resistance to “polyvalent Pyobacteriophage purified” and to “Staphylococcal bacteriophage” 1.8 times (p = 0.041) and 1.5 times (p = 0.05) more often than oral cultures. Staphylococcus haemolyticys strains from intestinal contents were 2.4 times more likely to be resistant to “Staphylococcal bacteriophage” (p = 0.032), 3 times more likely to “Intestinal bacteriophage” (p = 0.026), and pharyngeal isolates 1.6 times more likely to show resistance to “polyvalent Pyobacteriophage” (p = 0.042) compared to intestinal strains. Staphylococcus aureus isolated from various biomaterials had no differences in the frequency and spectrum of bacteriophage resistance (p > 0.05). Conclusion. Staphylococci isolated from the microbiota of medical staff are characterized by a high frequency of resistance to commercial bacteriophages. The spectrum of resistance to bacteriophages in strains isolated from the oropharynx and intestines differs.

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