Strength features in diabetic rats after repeated fracture of the tibia and treatment of alterations by means systemic and local calcium sources

Diabetes exerts negative effects on different organs and systems including skeletal system. Diabetes features increase of risk of fractures and healing of such fractures is also complicated. However, the effects of fracture consequences of repeated fracture on bone strength is not well known. Aim. To test strength features of the tibia in diabetic rats after repeated fracture and to consider administration of systemic and local calcium sources for treatment purposes. Material and methods. The study involved 168 female rats with hyperglycemia and obesity. Repeated fracture was modeled as formation of consecutive openings in the femur and the tibia. Fracture of the tibia was modeled after healing of the femur fracture healing. After formation of the defect in the tibia, in several animals the defect remained empty and in other animals the defect was filled with biogenic hydroxyapatite implants (OK-015). A group of animals with empty openings received intragastric calcium drug Calcemin Advance in therapeutic dosage. Another group of animals with the implants also received intragastric Calcemin Advance. Results. Diabetic animals with empty defects exhibited deranged strength features without restoration signs up to the end of observation. In animals that received Calcemin Advance, restoration of strength was observed in later terms mostly due to restoration of mineral contents however constructional features began recovering int the beginning of observation. In animals with hydroxyapatite implants constructional features worsened yet tissue strength increased. Application of both Calcemin Advance and OK-015 results in restoration of all strength features due to systemic and local administration of calcium sources. Conclusion. Repeated fracture in animals with type 2 diabetes results in considerable derangement of strength of the tibia. Administration of Calcemin Advance and implantation of hydroxyapatite material result in restoration of strength feature of the bone. Combined action of intragastric Calcemin Advance and hydroxyapatite implants appears to be the most optimal for recovery of the tibia strength.

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