Characteristic of gut microbiota in young people in Arkhangelsk

The study of the biodiversity of the colon microbiota in residents of the Arctic regions is currently of particular interest. The combination of cultural and molecular genetic methods allows for a comprehensive assessment of the microbiota spectrum. Purpose of the study – to assess the colon microbiota in young, healthy residents of Arkhangelsk. The material for the study was feces from healthy residents (n = 43). Analysis of the nature of the microbiota was carried out using cultural methods and PCR with the determination of 33 indicators. Research results. According to PCR data, 100 % of the subjects had a deficiency of lactobacilli; Roseburia inulinivorans was present in 20.9 %, Blautia spp. in 72.1 %, Agathobacter rectalis in 37.2 %, E. coli in 60.46 %, and there was an excess of Fusobacterium nucleatum in 16.28% of cases, Streptococcus spp. in 13.95 %, Acinetobacter spp. in 6.97 %, and opportunistic representatives of the order Enterobacteriales in 32.58 %. According to the results of the cultural research method, a deficiency of lactobacilli was recorded in 97.67 % of cases, enterococci in 51.16 %, Bacteroides in 27.91 %, typical (lactose +) E. coli in 72.09 %, bifidobacteria in 13.95 %, as well as detection of an excess of Klebsiella spp. in 32.58 % and Staphylococcus aureus in 4.65%. The average amount of lactobacilli by the cultural method was 4.81 lg CFU/g, Enterococci 5.0 lg CFU/g, S. aureus 5.12 lg CFU/g, K. pneumoniae 4.75 lg CFU/g, K. oxytoca 5.62 lg CFU/g, E. coli 6.52 lg CFU/g, Bifidobacterium spp. 9.0 lg CFU/g, and Bacteroides spp. 9.0 lg CFU/g. Statistically significant differences in the identification of microorganisms were found for Lactobacillus spp., Enterococcus spp., Bifidobacterium spp., and Bacteroides spp. (p < 0.001). An imbalance of the colon microbiota in healthy residents of Arkhangelsk was revealed through cultural and molecular genetic research methods.

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