BACTERICIDAL PROPERTIES OF XEROGEL BASED ON NEUTRAL HYDROSOL TiO2 FOR LOCAL THERAPY OF BURNS IN VIVO AND IN VITRO

Abstract

In the sunset era of antibiotics, the relevance of creating dosage forms that have antibacterial activity is increasing. Antiburn therapy is one of the main consumers of compounds with local antibacterial activity. In this work, we studied the anhydrous form of the gel, a xerogel based on a neutral hydrosol of titanium dioxide, which is an alternative to hydrogels when creating wound coverings. Staphylococcus was selected as the test object for the study of antimicrobial activity. Staphylococcus aureus 209 P and Escherichia coli 292-116. The antibacterial properties of the drug were investigated by diffusion methods in agar and cell suspensions. In addition, an experiment was conducted on 30 Wistar rats to form anIIIB degree burn in the interscapular region. The animals were divided into 3 groups: the control group without treatment, the comparison group animals treated with argosulfan and the experimental group in which aluminium xerogel was applied to the animals. The number of colonies forming units (CFU) in 1 ml of separated wounds was established on days 1, 3, 7, and 14 after modelling by counting macrocolonies grown on agarized and semi agarized growth media. It is shown that the drug has pronounced antibacterial properties against grampositive and gramnegative types of bacteria. The greatest effect on microorganisms can be achieved by diluting the drug no more than 1:1. bactericidal activity is based on the photocatalytic effect potentiated by the mesoporous structure of the xerogel. The skin application method, the use of a xerogel based on a neutral hydrosol of titanium dioxide as preparation for treating burn wounds, allows these concentrations to be achieved on the wound surface and does not involve significant dilution during use. Measurement data in an in vivo experiment demonstrate a prolonged antibacterial effect that increases treatment time.