CONTAMINATING MICROFLORA AT A TUBERCULOSIS TEST: DEPENDENCE ON CULTURE MEDIA FOR PRIMARY INOCULATION
https://doi.org/10.17021/2019.14.4.29.36
Abstract
Microbiological research on tuberculosis is a complex and strictly regulated process, the main purpose of which is the isolation and identification of mycobacteria of the tuberculosis complex. This process involves the use of a specific list of culture media, as well as the process of decontamination, aimed at suppressing the growth of concomitant bacterial microflora.The use of additional culture media for primary inoculation during the study for tuberculosis allows one to isolate not only classical “contaminants” from the clinical material, but also microflora, which has clinical significance in certain groups of patients.The results obtained in the study allow us to conclude that representatives of acid-resistant actinomycetes stand out in the structure of the contaminating microflora that grew on liquid media much more often. Among which were isolated non-tuberculous mycobacteria with atypical cultural properties, as well as representatives of the genera Nocardia spp., Gordonia spp., Rhodococcus spp., Tsukamurella spp.
References
1. Пунга, В. В. Контроль ситуации по туберкулезу на территориях Российской Федерации, курируемых ФГБНУ «Центральный НИИ туберкулеза», за 2014-2015 гг. / В. В. Пунга, М. А. Якимова, Т. В. Измайлова, Л. И. Русакова, В. В. Тестов // Туберкулез и болезни легких. - 2016. - Т. 94, № 9. - С. 11-17.
2. Севастьянова, Э. В. Оценка комплекса микробиологических и молекулярно-генетических методов исследований для диагностики туберкулеза / Э. В. Севастьянова, В. А. Пузанов, Т. Г. Смирнова, Е. Е. Ларионова, Л. Н. Черноусова // Туберкулез и болезни легких. - 2015. - № 1. - С. 35-41.
3. Aggarwal, D. Pulmonary nocardiosis revisited a case series / D. Aggarwal, K. Garg, J. Chander, V. Saini, A. K. Janmeja // Lung India. - 2015. - Vol. 32, № 2. - P. 165-168.
4. Agterof, M. J. Nocardiosis : a case series and a mini review of clinical and microbiological features / M. J. Agterof, T. Van der Bruggen, M. Tersmette, E. J. Ter Borg, J. M. Van den Bosch, D. H. Biesma // Neth. J. Med. - 2007. - Vol. 65, № 6. - P. 199-202.
5. Alavi Darazam, I. Nocardiosis : risk factors, clinical characteristics and outcome / I. Alavi Darazam, M. Shamaei, M. Mobarhan, S. Ghasemi, P. Tabarsi, M. Motavasseli, D. Mansouri // Iran Red Crescent Med. J. - 2013. - Vol. 15, № 5. - P. 436-439.
6. Ambesh, P. Sternal osteomyelitis by GordoniaBronchialis in an immunocompetent patient after open heart surgery / P. Ambesh, A. Kapoor, D. H. Kazmi, M. Elsheshtawy, V. Shetty, Y. S. Lin, S. Kamholz // Ann. Card. Anaesth. - 2019. - Vol. 22, № 2. - P. 221-224.
7. Brown-Elliott, B. A. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy / B. A. Brown-Elliott, J. M. Brown, P. S. Conville, R. J. Wallace // Clin. Microbiol. Rev. - 2006. - Vol. 19, № 2. - P. 259-282.
8. Canterino, J. Nocardia thailandica Pulmonary Nocardiosis in a Post-Solid Organ Transplant Patient / J. Canterino, A. Paniz-Mondolfi, B. Brown-Elliott, W. Vientos, R. Vasireddy, R. Wallace, S. Campbell // J. Clin. Microbiol. - 2015. - Vol. 53, № 11. - P. 3686-3690.
9. Chatterjee, M. Effects of different methods of decontamination for successful cultivation of Mycobacterium tuberculosis / M. Chatterjee, S. Bhattacharya, K. Karak // Indian J. Med. Res. - 2013. - Vol. 138, № 4. - P. 541-548.
10. Chen, C. H. Tsukamurella tyrosinosolvens bacteremia with coinfection of Mycobacterium bovis pneumonia : case report and literature review / C. H. Chen, C. T. Lee, T. C. Chang // Springer Plus. - 2016. - Vol. 5, № 1. - P. 2033.
11. Conville, P. S. The complexities of nocardia taxonomy and identification / P. S. Conville, B. A. Brown-Elliott, T. Smith, A. M. Zelazny // J. Clin. Microbiol. - 2017. - Vol. 56, № 1. - P. 1417-1419.
12. Gao, B. Phylogenetic framework and molecular signatures for the main clades of the phylum Actinobacteria. Microbiol / B. Gao, R. Gupta // Mol. Biol. Rev. - 2012. - Vol. 76. - P. 66-112.
13. Kandi, V. Human Nocardia Infections : A Review of Pulmonary Nocardiosis / V. Kandi // Cureus. - 2015. - Vol. 7, № 8. - P. e304.
14. Maraki, S. Primary lymphocutaneous nocardiosis in an immunocompetent patient / S. Maraki, S. Chochlidakis, E. Nioti, Y. Tselentis // Ann. Clin. Microbiol. Antimicrob. - 2004. - Vol. 3. - Р. 24.
15. Marín, M. Identification of Nocardia species from clinical isolates using MALDI-Tof mass spectrometry / M. Marín, A. Ruiz, C. Iglesias, L. Quiroga, E. Cercenado, P. Martín-Rabadán, E. Bouza, B. Rodríguez-Sánchez // Clin. Microbiol. Infect. - 2018. - Vol. 24, № 12. - P. 1342.
16. Nishi, S. Emerging bacterial, fungal, and viral respiratory infections in transplantation / S. Nishi, G. Valentine, S. Duncan // Infect. Dis. Clin. North Am. - 2010. - Vol. 24, № 3. - P. 541-555.
17. Simner, P. J. Mycobacterium and aerobic actinomycete culture : are two medium types and extended incubation times necessary? / P. J. Simner, K. A. Doerr, L. K. Steinmetz, N. L. Wengenack // J. Clin. Microbiol. - 2016. - Vol. 54, № 4. - P. 1089-1093.
18. Steinbrink, J. Manifestations and outcomes of nocardia infections : Comparison of immunocompromised and nonimmunocompromised adult patients / J. Steinbrink, J. Leavens, C. A. Kauffman, M. H. Miceli // Medicine. - 2018. - Vol. 97, № 40. - P. E12436.
19. Vazquez-Boland, J. Rhodococcus equi : the many facets of a pathogenic actinomycete / J. Vazquez-Boland // Vet. Microbiol. - 2013. - Vol. 167, № 1-2. - P. 9-33.
20. Werno, A. M. Recurrent breast abscess caused by Gordoniabronchialis in an immunocompetent patient / A. M. Werno, T. P. Anderson, S. T. Chambers, H. M. Laird, D. R. Murdoch // J. Clin. Microbiol. - 2005. - Vol. 43, № 6. - P. 3009-3010.
21. Yu, M. C. Evaluation of the Rapid MGIT TBc Identification Test for Culture Confirmation of Mycobacterium tuberculosis Complex Strain Detection / M. C. Yu, H. Y. Chen, M. H. Wu, W. L. Huang, Y. M. Kuo, F. L. Yu, R. Jou // J. Clin. Microbiol. - 2011. - Vol. 49, № 3. - P. 802-807.
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For citations:
Lyamin A.V. CONTAMINATING MICROFLORA AT A TUBERCULOSIS TEST: DEPENDENCE ON CULTURE MEDIA FOR PRIMARY INOCULATION. Astrakhan medical journal. 2019;14(4):29-36. (In Russ.) https://doi.org/10.17021/2019.14.4.29.36
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