ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF EXTRACTS OF VAGINAL ISOLETS OF CORYNEBACTERIUM AMYCOLATUM

Abstract. A serious global problem of the 21st century is the fight against bacterial infections caused by microorganisms with multiple drug resistance. Scientists are constantly searching for new therapeutic agents active against antibiotic-resistant strains. Secondary metabolites of microbial origin have historically proven their importance as a source of valuable compounds with antimicrobial activity. The genus Corynebacterium spp., which has recently attracted the attention of scientists due to the discovery of individual species with probiotic potential, has not been previously studied in this aspect. Purpose of the study: to study the antimicrobial and antibiofilm activity of extracts of vaginal isolates of Corynebacterium amycolatum. Material and research methods. The material for the study were extracts of three vaginal isolates of C. amycolatum ICIS 5, ICIS 9 and ICIS 53. The antimicrobial activity of the extracts was studied in vitro against 4 test strains of pathogenic microorganisms using the paper disk method. The effect of the extracts on pre-formed biofilms of the test strains was studied in 96-well polystyrene plates. The morphology of the test strain biofilms after pre-treatment with C. amycolatum extracts was studied using scanning electron microscopy. The experimental data were processed using variation statistics methods with the calculation of the arithmetic mean and its error (M±m) from 3 measurements. Research results. The antibacterial and antibiofilm activity of all tested C. amycolatum extracts was established against Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Enterococcus faecium ATCC 19434. The severity of antibacterial activity and the degree of destruction of the formed biofilm depended on the type of the tested microorganism. Scanning electron microscopy studies revealed a flat, scattered and unstructured architecture of the biofilms of the test strains. Conclusion. The data obtained open the prospect of studying the metabolic profile of C. amycolatum extracts to understand the nature and mechanism of the detected antibacterial and antibiofilm activity.

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