astmedАстраханский медицинский журналAstrakhan medical journal1992-6499ФГБОУ ВО Астраханский ГМУ Минздрава России10.17021/2020.15.4.8.15astmed-93Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL INVESTIGATIONSNOVEL APPROACH FOR ISOLATION OF LYTIC BACTERIOPHAGES AGAINST STAPHYLOCOCCUS AUREUSRubalskiiE. O.Rubalskii.Evgenii@mh-hannover.deBashkinaO. A.bashkina1@mail.ruHaverichA.Haverich.Axel@mh-hannover.deAleshkinA. V.ava@gabri.ruSamotruevaM. A.ms1506@mail.ruPopovE. A.agma@astranet.ruKuehnC.Kuehn.Christian@mh-hannover.deAbdrakhmanovaR. O.radmilaazo@mail.ruDosmukhanovaE. G.ametist07@mail.ruRuemkeS.Ruemke.Stefan@mh-hannover.deSalmoukasC.Salmoukas.Christina@mh-hannover.deRubalskyM. O.m.o.rubalsky@gmail.com.Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School; Laboratory of Applied Immunochemistry, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and MicrobiologyRussian FederationAstrakhan State Medical UniversityRussian FederationDepartment of Cardiothoracic, Transplantation and Vascular Surgery; Hannover Medical SchoolRussian FederationLaboratory of Clinical Microbiology and Biotechnology of Bacteriophages, G.N. Gabrichevsky Moscow Research Institute for Epidemiology and MicrobiologyRussian FederationCardiothoracic and Transplant Surgeon, Consultant, Leading Surgeon of Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical SchoolRussian FederationDepartment of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical SchoolRussian FederationCity Diagnostic LaboratoryRussian Federation202027022023154815Copyright © Rubalskii E.O., Bashkina O.A., Haverich A., Aleshkin A.V., Samotrueva M.A., Popov E.A., Kuehn C., Abdrakhmanova R.O., Dosmukhanova E.G., Ruemke S., Salmoukas C., Rubalsky M.O., 20232023Rubalskii E.O., Bashkina O.A., Haverich A., Aleshkin A.V., Samotrueva M.A., Popov E.A., Kuehn C., Abdrakhmanova R.O., Dosmukhanova E.G., Ruemke S., Salmoukas C., Rubalsky M.O.Rubalskii E.O., Bashkina O.A., Haverich A., Aleshkin A.V., Samotrueva M.A., Popov E.A., Kuehn C., Abdrakhmanova R.O., Dosmukhanova E.G., Ruemke S., Salmoukas C., Rubalsky M.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.astmedj.ru/jour/article/view/93

Strictly lytic or virulent bacteriophages against Staphylococcus aureus belong to order Caudovirales and comprise predominantly two genera: Kayvirus and Rosenblumvirus. Representatives of both taxonomic groups are well known as safe and viable antibacterial agents. Phages of the genus Kayvirus show usually a broad host range activity against clinical isolates of S. aureus. Nevertheless continuous isolation of new phages against these bacteria is required in order to provide sufficient activity for therapy of biofilms or to prevent phage inactivation by neutralizing antibodies. In the present study, we describe an approach for isolation of lytic bacteriophages of the genus Kayvirus by mitomycin C induction of clinical isolates of S. aureus. Our findings suggest the clinical isolates of S. aureus a reach source for lytic bacteriophages. However taking into account the inducibility of the Kayvirus representatives it have to be evaluated a presence of further unknown life cycles for these bacteriophages such as pseudolysogeny.

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The authors declare that there are no conflicts of interest present.