astmedАстраханский медицинский журналAstrakhan medical journal1992-6499ФГБОУ ВО Астраханский ГМУ Минздрава России10.17021/1992-6499-2025-2-105-114astmed-561Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL INVESTIGATIONSАнтибактериальная и антибиопленочная активность экстрактов вагинальных изолятов Corynebacterium amycolatumAntibacterial and antibiofilm activity of extracts of vaginal isolates of Corynebacterium amycolatumhttps://orcid.org/0000-0001-6231-7028ГладышеваИ. В.GladyshevaI. V.

Ирина Вячеславовна Гладышева   - кандидат медицинских наук, старший научный сотрудник лаборатории биомедицинских технологий.

Оренбург

Irina V. Gladysheva - Cand. Sci. (Med.), Senior Researcher, Laboratory of Biomedical Technologies.

Orenburg

gladishevaiv@yandex.ruhttps://orcid.org/0000-0002-0707-2977ЧеркасовС. В.CherkasovS. V.

Сергей Викторович Черкасов - доктор медицинских наук, академик Российской академии наук, главный научный сотрудник лаборатории биомедицинских технологий.

Оренбург

Sergey V. Cherkasov - Dr. Sci. (Med.), Academician of the Russian Academy of Sciences, Chief Researcher of the Laboratory of Biomedical Technologies.

Orenburg

cherkasovsv@yandex.ruИнститут клеточного и внутриклеточного симбиоза Уральского отделения РАН – структурное научное подразделение Оренбургского федерального исследовательского центраРоссияInstitute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS – a structural scientific unit of the Orenburg Federal Research CenterRussian Federation202517072025202105113Copyright © Гладышева И.В., Черкасов С.В., 20252025Гладышева И.В., Черкасов С.В.Gladysheva I.V., Cherkasov S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.astmedj.ru/jour/article/view/561

Глобальной мировой проблемой XXI века является борьба с бактериальными инфекциями, вызванными микроорганизмами со множественной лекарственной устойчивостью. Учеными постоянно ведутся поиски новых терапевтических средств, активных в отношении антибиотикорезистентных штаммов. Вторичные метаболиты микробного происхождения исторически доказали свою значимость в качестве источника ценных соединений с антимикробной активностью. Род Corynebacterium spp., который в последнее время привлекает внимание исследователей благодаря открытию отдельных видов с пробиотическим потенциалом, ранее не был изучен в данном аспекте.

Цель исследования

Цель исследования. Изучение антимикробной и антибиопленочной активности экстрактов трех вагинальных изолятов Corynebacterium amycolatum.

Материалы и методы

Материалы и методы. Материалом для исследования послужили экстракты трех вагинальных изолятов C. amycolatum ICIS 5, ICIS 9 и ICIS 53. Антимикробную активность экстрактов изучали в условиях in vitro в отношении 4 тест-штаммов патогенных микроорганизмов с помощью метода бумажных дисков. Исследование влияния экстрактов на предварительно сформированные биопленки тест-штаммов проводили в 96-луночных полистироловых планшетах. Морфологию биопленок тест-штаммов после предварительной обработки экстрактами C. amycolatum изучали с помощью сканирующей электронной микроскопии. Экспериментальные данные были обработаны методами вариационной статистики с вычислением из трех измерений средней арифметической и ее ошибки (M ± m).

Результаты исследования

Результаты исследования. Установлена антибактериальная и антибиопленочная активность всех тестируемых экстрактов C. amycolatum в отношении Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa АТСС 27853, Staphylococcus aureus ATCC 25923 и Enterococcus faecium ATCC 19434. Выраженность антибактериальной активности и степень разрушения сформированной биопленки зависела от вида тестируемого микроорганизма. Исследования, выполненные с помощью сканирующей электронной микроскопии, выявили плоскую, разбросанную и неструктурированную архитектуру биопленок тест-штаммов.

Заключение

Заключение. Полученные данные открывают перспективу изучения метаболического профиля экстрактов C. amycolatum для понимания природы и механизма обнаруженного антибактериального и антибиопленочного действия.

A serious global problem of the 21st century is the fight against bacterial infections caused by microorganisms with multiple drug resistance. Scientists are constantly searching for new therapeutic agents active against antibiotic-resistant strains. Secondary metabolites of microbial origin have historically proven their importance as a source of valuable compounds with antimicrobial activity. The genus Corynebacterium spp., which has recently attracted the attention of scientists due to the discovery of individual species with probiotic potential, has not been previously studied in this aspect.

Purpose of the study

Purpose of the study: to study the antimicrobial and antibiofilm activity of extracts of vaginal isolates of Corynebacterium amycolatum.

Material and methods

Material and methods. The material for the study were extracts of three vaginal isolates of C. amycolatum ICIS 5, ICIS 9 and ICIS 53. The antimicrobial activity of the extracts was studied in vitro against 4 test strains of pathogenic microorganisms using the paper disk method. The effect of the extracts on pre-formed biofilms of the test strains was studied in 96-well polystyrene plates. The morphology of the test strain biofilms after pre-treatment with C. amycolatum extracts was studied using scanning electron microscopy. The experimental data were processed using variation statistics methods with the calculation of the arithmetic mean and its error (M±m) from 3 measurements.

Research results

Research results. The antibacterial and antibiofilm activity of all tested C. amycolatum extracts was established against Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Enterococcus faecium ATCC 19434. The severity of antibacterial activity and the degree of destruction of the formed biofilm depended on the type of the tested microorganism. Scanning electron microscopy studies revealed a flat, scattered and unstructured architecture of the biofilms of the test strains.

Conclusion

Conclusion. The data obtained open the prospect of studying the metabolic profile of C. amycolatum extracts to understand the nature and mechanism of the detected antibacterial and antibiofilm activity.

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The authors declare that there are no conflicts of interest present.